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  • ilya.kuzovkin
    Junior Member
    • Jan 2013
    • 2

    #1

    Problem with Bioconductor ArrayExpressHTS package

    Hi,

    I'm trying to make use of ArrayExpressHTS R package, but I hit the wall in the very beginning.

    I used this tutorial An Introduction to ArrayExpressHTS.

    And this is my code:

    Code:
    # Set up external tools
    options('ArrayExpressHTS.fasta_formatter' = '/Applications/Bioinformatics/fastx_toolkit')
    options('ArrayExpressHTS.cufflinks' = '/Applications/Bioinformatics/cufflinks')
    options('ArrayExpressHTS.samtools' = '/opt/local/bin')
    options('ArrayExpressHTS.bwa' = '/Applications/Bioinformatics/bwa')
    options('ArrayExpressHTS.mmseq' = '/Applications/Bioinformatics/mmseq')
    options('ArrayExpressHTS.bam2hits' = '/Applications/Bioinformatics/mmseq')
    options('ArrayExpressHTS.bowtie' = '/opt/local/bin')
    options('ArrayExpressHTS.tophat' = '/opt/local/bin')
    
    # Load libraries
    library("ArrayExpressHTS")
    
    # Get data
    aehts <- ArrayExpressHTS("E-GEOD-16190")
    The problem is that I get some kind of unknown error from ArrayExpressHTS function:
    Code:
    > aehts <- ArrayExpressHTS("E-GEOD-16190", dir = expfolder)
    Fri Jan 18 18:20:21 2013 [AEHTS]  Not Found.
    Error in ArrayExpressHTS("E-GEOD-16190") :
    Worst part is that it does not say what went wrong, just "Error in ArrayExpressHTS ..." and nothing else.
  • ilya.kuzovkin
    Junior Member
    • Jan 2013
    • 2

    #2
    Turned out to be stupid question.
    The answer is that I did not specify reference directory.
    This works fine:
    Code:
    aehts <- ArrayExpressHTS("E-GEOD-16190", refdir='reference')

    Comment

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