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  • Kotoro
    Member
    • May 2011
    • 31

    #1

    problem with picard fixmateinfo

    command used in a function defined in a BASH script:
    Code:
    java -Xmx$JAVA_RAM -Xms$JAVA_RAM -jar /UCHC/HPC/Everson_HPC/picard/bin/FixMateInformation.jar \
    MAX_RECORDS_IN_RAM=$MRECORDS \
    I=./GATK_prep/$1.addReadGroups \
    O=./GATK_prep/$1.fixMateInfo
    exception that resulted:
    Code:
    grep Exception qsub_logs/SKA091711TCBL5B.step2-6.*
    qsub_logs/SKA091711TCBL5B.step2-6.err:Exception in thread "main" net.sf.samtools.util.RuntimeIOException: java.io.IOException: No space left on device
    qsub_logs/SKA091711TCBL5B.step2-6.err:Caused by: java.io.IOException: No space left on device
    qsub_logs/SKA091711TCBL5B.step2-6.err:##### ERROR MESSAGE: Couldn't read file /UCHC/Everson/Projects/Bladder/Pt5/B/SKA091711TCBL5B.4GATK.recal.bam because java.io.FileNotFoundException: SKA091711TCBL5B.4GATK.recal.bam (No such file or directory)
    qsub_logs/SKA091711TCBL5B.step2-6.err:##### ERROR MESSAGE: Couldn't read file /UCHC/Everson/Projects/Bladder/Pt5/B/SKA091711TCBL5B.4GATK.recal.bam because java.io.FileNotFoundException: SKA091711TCBL5B.4GATK.recal.bam (No such file or directory)
    I have recently freed almost 7 TB of data that we had been storing on this cluster's user share (which is approximately 84T in size and only 75% used). The run on this file is not producing enough data to warrant hitting any user-based quotas that I would not have already encountered previously if they had been in place. In fact I can create many duplicate copies of large files manually with no disk usage warnings.

    And yet picard's tool claims it is running out of disk space. Whats wrong? How do I get past this error?
    Last edited by Kotoro; 01-23-2013, 10:04 AM.
  • FractalExpression
    Member
    • Feb 2011
    • 12

    #2
    I would explicitly declare a scratch directory, as I've had this issue in the past with FixMateInfo:

    Code:
    -Djava.io.tmpdir= /your_scratch_directory
    Also, somewhat redundant, but just to be safe add:

    Code:
    TMP_DIR=/your_scratch_directory
    Good luck.
    Petri Dish Talk

    Comment

    • iyer0000
      Junior Member
      • Sep 2014
      • 1

      #3
      even i have problem with this posts,please move to stack underflow

      Comment

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