Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Kotoro
    Member
    • May 2011
    • 31

    #1

    problem with picard fixmateinfo

    command used in a function defined in a BASH script:
    Code:
    java -Xmx$JAVA_RAM -Xms$JAVA_RAM -jar /UCHC/HPC/Everson_HPC/picard/bin/FixMateInformation.jar \
    MAX_RECORDS_IN_RAM=$MRECORDS \
    I=./GATK_prep/$1.addReadGroups \
    O=./GATK_prep/$1.fixMateInfo
    exception that resulted:
    Code:
    grep Exception qsub_logs/SKA091711TCBL5B.step2-6.*
    qsub_logs/SKA091711TCBL5B.step2-6.err:Exception in thread "main" net.sf.samtools.util.RuntimeIOException: java.io.IOException: No space left on device
    qsub_logs/SKA091711TCBL5B.step2-6.err:Caused by: java.io.IOException: No space left on device
    qsub_logs/SKA091711TCBL5B.step2-6.err:##### ERROR MESSAGE: Couldn't read file /UCHC/Everson/Projects/Bladder/Pt5/B/SKA091711TCBL5B.4GATK.recal.bam because java.io.FileNotFoundException: SKA091711TCBL5B.4GATK.recal.bam (No such file or directory)
    qsub_logs/SKA091711TCBL5B.step2-6.err:##### ERROR MESSAGE: Couldn't read file /UCHC/Everson/Projects/Bladder/Pt5/B/SKA091711TCBL5B.4GATK.recal.bam because java.io.FileNotFoundException: SKA091711TCBL5B.4GATK.recal.bam (No such file or directory)
    I have recently freed almost 7 TB of data that we had been storing on this cluster's user share (which is approximately 84T in size and only 75% used). The run on this file is not producing enough data to warrant hitting any user-based quotas that I would not have already encountered previously if they had been in place. In fact I can create many duplicate copies of large files manually with no disk usage warnings.

    And yet picard's tool claims it is running out of disk space. Whats wrong? How do I get past this error?
    Last edited by Kotoro; 01-23-2013, 10:04 AM.
  • FractalExpression
    Member
    • Feb 2011
    • 12

    #2
    I would explicitly declare a scratch directory, as I've had this issue in the past with FixMateInfo:

    Code:
    -Djava.io.tmpdir= /your_scratch_directory
    Also, somewhat redundant, but just to be safe add:

    Code:
    TMP_DIR=/your_scratch_directory
    Good luck.
    Petri Dish Talk

    Comment

    • iyer0000
      Junior Member
      • Sep 2014
      • 1

      #3
      even i have problem with this posts,please move to stack underflow

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
        by SEQadmin2




        NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
        Today, 06:46 AM
      • SEQadmin2
        Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
        by SEQadmin2



        Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

        Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
        ...
        10-14-2026, 09:55 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 10-28-2026, 09:57 AM
      0 responses
      24 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-28-2026, 09:51 AM
      0 responses
      15 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-22-2026, 11:13 AM
      0 responses
      70 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-22-2026, 10:48 AM
      0 responses
      48 views
      0 reactions
      Last Post SEQadmin2  
      Working...