I have illumina RNASeq data for of 3 samples each with untreated and treated conditions. Thus i have three biological replicates for each conditions. I am not expecting much change after the 'treatment'; What parameters of DESeq and Cuffdiff do I need to tweak to handle conditions with more 'similarity' than 'difference' ? what normalization method should be used in cuffdiff2 , the default geometric mean normalisation, upper quartile normalisation (-N) or --raw-mapped-norm ?
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM