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I am trying to assemble paired read illumina data using the config file:
[LIB]
max_rd_len=100
asm_flags=3
rank=1
q1= JaneD_GCCAAT_L006_R1_noadap_001_Asssss.fastq_filtered_trimmed
q2= JaneD_GCCAAT_L006_R2_noadap_001_Asssss.fastq_filtered_trimmed
However I get an error saying it can't read the files. the files are fastq files and are not corrupted as I have used the same files with no issues on trinity.
The only thing I can think of is that some of the quality lines start with a @, would this cause an issue? if so is there an alternative symbol I could use instead of the quality symbol @ without affecting my assembly?
[LIB]
max_rd_len=100
asm_flags=3
rank=1
q1= JaneD_GCCAAT_L006_R1_noadap_001_Asssss.fastq_filtered_trimmed
q2= JaneD_GCCAAT_L006_R2_noadap_001_Asssss.fastq_filtered_trimmed
However I get an error saying it can't read the files. the files are fastq files and are not corrupted as I have used the same files with no issues on trinity.
The only thing I can think of is that some of the quality lines start with a @, would this cause an issue? if so is there an alternative symbol I could use instead of the quality symbol @ without affecting my assembly?
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