Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • Farhat
    Member
    • Apr 2008
    • 21

    Using pbShort

    Hi,

    Did anyone have success using pbShort? I have tried several real and sample datasets and it runs without error but always get an empty tam file.

    -Farhat
    Farhat Habib
  • monsanto
    Junior Member
    • Oct 2008
    • 2

    #2
    Use pbShort

    Hi,

    I use the pbShort successfully ...
    What is the sequence of commands and their input parameters that you use at work?

    Monsanto

    Comment

    • ehscholl
      Junior Member
      • Nov 2008
      • 3

      #3
      pbShort keeps crashing on me

      I've been trying for about a week to compare a set of about 250k 454 reads to a reasonably small genome (54MB) and the pbShort program keeps crashing on me with an error message of:

      terminate called after throwing an instance of 'std:ut_of_range'
      what(): basic_string::substr


      I've sent an email in to Murth Labs about this but gotten no response. (To be fair, it's been about 24 hours since I sent the message...)

      Has anyone else seen this error before?

      TIA

      Comment

      • ehscholl
        Junior Member
        • Nov 2008
        • 3

        #4
        That last post should have been std :: out of range. I didn't realize it would automatically emoticon me

        Comment

        Latest Articles

        Collapse

        • GATTACAT
          Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
          by GATTACAT
          Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
          07-01-2026, 11:43 AM
        • SEQadmin2
          Nine Things a Sample Prep Scientist Thinks About Before Sequencing
          by SEQadmin2


          I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

          Here are nine questions we think about, in roughly the order they matter, before...
          06-18-2026, 07:11 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by SEQadmin2, Yesterday, 11:08 AM
        0 responses
        7 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 06-30-2026, 05:37 AM
        0 responses
        11 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 06-26-2026, 11:10 AM
        0 responses
        19 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 06-17-2026, 06:09 AM
        0 responses
        53 views
        0 reactions
        Last Post SEQadmin2  
        Working...