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  • desse
    Junior Member
    • Sep 2012
    • 4

    #1

    bwa 0.6.2 -sampe command does not work.

    Hi,

    I use bwa to align sequence data (Paired End Data from Ilumina).

    I used bwa 0.6.2 but get stucked.

    Here is what I have done so far:

    Index:

    bwa index -a is allchr.fa
    (I got
    .amb
    .ann
    .bwt
    .fai
    .pac
    .sa)

    Alignment: pc_n.sh program

    REFERENCE=/Users/referenceData/chrall.fa
    $BWA aln -t 8 $REFERENCE $QUERY1 > $3_1.sai
    $BWA aln -t 8 $REFERENCE $QUERY2 > $3_2.sai
    $BWA sampe $REFERENCE $3_1.sai $3_2.sai $QUERY1 $QUERY2 > $3_out.sam

    And here is my Problem(message on terminal):

    [main] Version: 0.6.2-r126
    [main] CMD: /usr/bin/bwa sampe /Users/referenceData/chrall.fa mi_1.sai mi_2.sai q1.fq q2.fq
    [main] Real time: 43.905 sec; CPU: 42.962 sec
    /Users/pc_n.sh: line 44: mi_out.sam: command not found

    /Users/pc_n.sh: line 44 is "$BWA sampe $REFERENCE $3_1.sai $3_2.sai $QUERY1 $QUERY2 > $3_out.sam"

    This program worked using 0.5.9 version in the other PC.

    Please help me.
    Last edited by desse; 02-18-2013, 06:44 PM. Reason: delete a icon

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