Hi there 'RNA-sequers',
I am pretty new to this field and I would like to ask (something maybe basic) if anyone could please explain to me why you'd use a strand-specific protocol to build cDNA libraries out of total RNA instead of normal protocol (i.g., non strand specific one). I am struggling to see what are the benefits for using each, and definitely struggling to understand the biology foundation behind. I mean, you won't see more gene expression depending whether you use a strand specific protocol, right???.
My organism (petunia) does not have a reference genome, would a strand-specific protocol be a better fit???
Thanks very much!!.
Gonzalo.
I am pretty new to this field and I would like to ask (something maybe basic) if anyone could please explain to me why you'd use a strand-specific protocol to build cDNA libraries out of total RNA instead of normal protocol (i.g., non strand specific one). I am struggling to see what are the benefits for using each, and definitely struggling to understand the biology foundation behind. I mean, you won't see more gene expression depending whether you use a strand specific protocol, right???.
My organism (petunia) does not have a reference genome, would a strand-specific protocol be a better fit???
Thanks very much!!.
Gonzalo.
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