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  • Andrew73
    Junior Member
    • Mar 2013
    • 1

    #1

    Web Services Blastn Problems

    Hi, I've just started trying out the WSDL web services and I'm trying to implement a workflow for the NCBI Blastn web service.

    I know the contigs that I want to blast are available and on the nt database however, I keep getting incorrect database errors when trying to run.

    The only documentation I can find on the database abbreviations of NCBI have been less than useful, but I thought nt would be pretty straight forward.

    Following this large problem, I set up an alternative by downloading the whole nt database from NCBI and ran a blastall on an AWS instance, which took over an hour and a half for a single contig.

    So I'm struggling to be honest and would appreciate anyone's insight or suggestions.

    thanks for reading!

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