Do I need to run trim galore even if my reads are of good quality?

[genomics_search]

Hi,

I am a beginner in the field of genomics. So I have one doubt should I use trim galore to trim my sequence even if they are of good quality?

From the fastqc result I have I can say that all of them have "per base sequence quality" more than 29.

So is it required to run trim galore, just to remove the adapter sequence?

Thanks.

[fkrueger]

I guess this would depend on the type of experiment at hand, as some applications are a bit more forgiving than others. If you wanted you could run Trim Galore (and or your alignment method of choice), look at the trimming (and/or alignment) stats and decide then whether it was worth it. Since long read lengths tend to coincide with adapter contamination we trim all sequences by default these days.