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  • Pennaki
    Junior Member
    • Dec 2012
    • 8

    #1

    Samtools on Mac: how to use??

    Hello everyone!!

    I'm very new to bioinformatics, trying to index a .bam file on my mac using samtools, so my question might be very silly. I installed samtools yesterday, tried to convert a .sam to .bam and it worked. However, today I got a .bam file which I tried to index it using the

    index test1.bam

    but I keep getting this:

    -bash: index: command not found

    What am I doing wrong?!

    Thank u!
  • chadn737
    Senior Member
    • Jan 2009
    • 392

    #2
    did you put "samtools" before "index"?

    Your command should be:

    Code:
    $ samtools index test1.bam

    Comment

    • Pennaki
      Junior Member
      • Dec 2012
      • 8

      #3
      I did, and I'm getting this:

      -bash: samtools: command not found

      Comment

      • chadn737
        Senior Member
        • Jan 2009
        • 392

        #4
        Are you sure you have samtools installed correctly?

        Comment

        • chadn737
          Senior Member
          • Jan 2009
          • 392

          #5
          How did you get samtools to convert a sam to a bam?

          Comment

          • Pennaki
            Junior Member
            • Dec 2012
            • 8

            #6
            By typing:

            $samtools view -bS test1.sam > test1.bam

            :/

            Comment

            • Pennaki
              Junior Member
              • Dec 2012
              • 8

              #7
              Got it! It had to do with the path or something, I typed ./samtools instead of samtools and it worked!!

              Comment

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