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  • Samtools on Mac: how to use??

    Hello everyone!!

    I'm very new to bioinformatics, trying to index a .bam file on my mac using samtools, so my question might be very silly. I installed samtools yesterday, tried to convert a .sam to .bam and it worked. However, today I got a .bam file which I tried to index it using the

    index test1.bam

    but I keep getting this:

    -bash: index: command not found

    What am I doing wrong?!

    Thank u!

  • #2
    did you put "samtools" before "index"?

    Your command should be:

    Code:
    $ samtools index test1.bam

    Comment


    • #3
      I did, and I'm getting this:

      -bash: samtools: command not found

      Comment


      • #4
        Are you sure you have samtools installed correctly?

        Comment


        • #5
          How did you get samtools to convert a sam to a bam?

          Comment


          • #6
            By typing:

            $samtools view -bS test1.sam > test1.bam

            :/

            Comment


            • #7
              Got it! It had to do with the path or something, I typed ./samtools instead of samtools and it worked!!

              Comment

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