Mutation Frequency vs. Depth of Coverage

AmpliSeqMan

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Join Date: Jul 2012

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Mutation Frequency vs. Depth of Coverage

04-02-2013, 12:28 PM

Tumors are composed of malignant cells, stromal cells and immune cells. Accordingly, a tumor with a heterozygous mutation may only have 40% cancer cellularity (60% non-cancer cells) and therefore (20% mutant alleles). Does anyone know the reference that determines the correct depth of coverage needed to be highly confident (>99%) that the mutation will be detected? Thanks!
Tags: depth of coverage, mutation frequency

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