I am currently working with assembly programs and most of the assemblers output max contig length and N50. But many papers also report percent of reference genome covered by the contigs in their results. So if I have a set of contigs and a reference genome then how can I measure the percent of reference genome covered by the contigs of length greater than say x. I used NUCMER but I can get a delta or coords file as an output but not the exact percentage. Anyone knows of any tool which can output this parameter?
-
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
