Hi,
I have two sets of samples sequenced using Illumina GA(II) and Illumina HiSeq. I would like to merge above two datasets so that I have more power to my differential expression analysis between two biological groups.
I am wondering whether the technical differences between two technologies (as shown in "Evaluation of genomic high-throughput sequencing data generated on Illumina HiSeq and Genome Analyzer systems") can be minimized or made comparable by a normal MDS scaling following a normalization by library sizes.
What are you opinions about that ?
Thanks in advance.
I have two sets of samples sequenced using Illumina GA(II) and Illumina HiSeq. I would like to merge above two datasets so that I have more power to my differential expression analysis between two biological groups.
I am wondering whether the technical differences between two technologies (as shown in "Evaluation of genomic high-throughput sequencing data generated on Illumina HiSeq and Genome Analyzer systems") can be minimized or made comparable by a normal MDS scaling following a normalization by library sizes.
What are you opinions about that ?
Thanks in advance.
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