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  • lre1234
    Senior Member
    • Aug 2011
    • 110

    #1

    GATK BaseRecalibrator -output bam

    Hi,
    I am doing my post-alignment processing on whole genomes (~40X) coverage. My aligned bam files are each ~100GB in size. After I go through all of the steps (indelrealigning, fixing mate-pairs, basereaclibrating, etc), my final output bam file after the BQSR print reads has dramatically jumped in size. They have gone from the ~100GB in size to over 120GB in size. Is this a normal occurrence and something to be alarmed by?
    Thanks

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