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  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #1

    Cufflinks v.2.1.1 on Rocks v.5.3

    We have a very specific problem with cufflinks v.2.1.1 on a Rocks cluster (v.5.3). We have been getting segmentation faults with cufflinks v.2.1.* releases.

    The error message is not very informative (even when run with -v option). Following is an excerpt from the test data run included in the Trapnell Nature Protocols paper (this example works fine with Cufflinks v. 2.0.2)

    Code:
    2L:7534-9404    Assessing overlaps between 1 fragments for identical conflict sets
    2L:7534-9404    Compressed 0 of 1 redundant fragments (0.000000 percent)
    2L:7534-9404    Ignoring 25 strictly contained fragments
    2L:7534-9404    Assembling bundle with 1 hits
    2L:7534-9404    Calculating scaffold densities
    2L:7534-9404    Creating compatibility graph
    2L:7534-9404    Performing final collapse round
    Extracted 1 contiguous transfrags from 1 scaffolds
    2L:7534-9404    Starting new collapse round
    line 8:  7316 Segmentation fault      cufflinks -p 4 --no-update-check -v -o GSM794483_C1_R1_clout_new GSM794483_C1_R1_thout/accepted_hits.bam
    Our system administrators have rebuilt the relevant cufflinks module (we use modules to manage software) multiple times with various Boost library/Eigen combinations. We are stumped at this point as to what to try next.

    Is there someone on this forum who has cufflinks v.2.1.1 working on Rocks (v.5.3)?
    Last edited by GenoMax; 06-04-2013, 10:03 AM.
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    It turned out that a newer version of GCC (v.4.8.1) was required to compile cufflinks to fix this problem.

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