Assembling against a reference

pari_89

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Assembling against a reference

06-27-2013, 06:58 AM

Hello everyone,

I have been doing some reading about mapping and assembling against a reference and I cannot quite understand the difference? What I understand it that mapping against a reference gives alignment scores while assembling produce a set of contigs.

I have used CLCworkbench - maps reads against a reference, runMapping from Newbler and MIra to do a mapping assembly? I get a list of contigs as well.

Some sources say that mapping is not assembly. Can anyone please explain to me or give me some papers to understand this please.

Thank you.
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swaraj

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06-27-2013, 07:24 AM

This should help.

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http://www.ncbi.nlm.nih.gov/pubmed/21897427
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