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  • arkilis
    replied
    Originally posted by JackieBadger View Post
    it means if you do not have all of your amplicon i.e fwd primer---target sequence---rev primer then the sequence is discarded.


    You means that some amplicon may not complete like the structure in the above image? THX

    Leave a comment:


  • JackieBadger
    replied
    it means if you do not have all of your amplicon i.e fwd primer---target sequence---rev primer then the sequence is discarded.

    Leave a comment:


  • arkilis
    started a topic Amplicon processing

    Amplicon processing

    "By default, amplicon processing discards all reads that fail to reach the reverse primer (using the ShortQuality filter)"

    Could anyone explain this in a little bit detail? Thanks!

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