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  • arkilis
    Senior Member
    • Jul 2013
    • 119
    #1

    Amplicon processing

    "By default, amplicon processing discards all reads that fail to reach the reverse primer (using the ShortQuality filter)"

    Could anyone explain this in a little bit detail? Thanks!
    Tags: amplicon
    • JackieBadger
      Senior Member
      • Mar 2009
      • 385
      #2
      it means if you do not have all of your amplicon i.e fwd primer---target sequence---rev primer then the sequence is discarded.

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        • arkilis
          Senior Member
          • Jul 2013
          • 119
          #3
          Originally posted by JackieBadger View Post
          it means if you do not have all of your amplicon i.e fwd primer---target sequence---rev primer then the sequence is discarded.


          You means that some amplicon may not complete like the structure in the above image? THX

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