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  • nimrod337
    Member
    • Jun 2013
    • 22

    #1

    More Samtools woes on mac

    Okay, I finally got samtools installed (or so I think.) after downloading Xcode, and getting the make command to work. But now when I try to use samtools, I keep getting:

    "-bash: samtools: command not found"

    several people had recommended adding the ./ before samtools to get it to work, but when I do that, I get this:

    "-bash: ./samtools: No such file or directory"

    So what's the deal?
    I need to be in the directory where samtools was installed, correct? so

    "cd XXXXX" where XXXXX is the directory that samtools was "make" into, then
    "./samtools faidx /Users/2/PGSC_DM_v3_2.1.11_pseudomolecules.fasta"

    This should compile an index using the .fasta file correct? Where am I going wrong here?
    I'm losing my mind here. I obviously don't have any UNIX background, but I get the jist, so if you could explain it too me as if I was your grandmother, that would really help.
    Thanks guys, this website has already been so helpful.
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    Just to confirm that you have built samtools correctly do this:

    "cd XXXXX" where XXXXX is the directory that samtools was "make" into
    and run the following command and post the output here

    Code:
    $ pwd
    $ ls -lt
    As long as "samtools" was correctly built the following command will work.

    Code:
    $ /[COLOR="Red"]replace_with_output_from_pwd_command_above[/COLOR]/samtools faidx /User/2/PGSC_DM_v3_2.1.11_pseudomolecules.fasta
    You can either go to the directory where you have samtools installed or you can run any of these programs from any other directory on your machine as long as you provide correct full (or relative) file paths.
    Last edited by GenoMax; 07-23-2013, 11:18 AM.

    Comment

    • dpryan
      Devon Ryan
      • Jul 2011
      • 3478

      #3
      If you have anyone locally in IT, you might just offer to buy him/her a beer or coffee for a quick bit of help. It'll make your life a bit easier going forward

      Comment

      • nimrod337
        Member
        • Jun 2013
        • 22

        #4
        Okay, I just cleared everything out of my folders, and made a new one, downloaded samtools again, made it again, and it seems to be working. Now I am just waiting on it to sort. Must have been a weird pathing thing... Seems to me all Unix problems are pathing related. Thank you for your help, and ON TO THE NEXT PROBLEM!! (Tablet)

        Comment

        • rhinoceros
          Senior Member
          • Apr 2013
          • 372

          #5
          Next you'll

          Code:
          cd to bamtools
          pwd
          cd
          nano .bash_profile
          and add the line
          Code:
          export PATH=/thePathPwdGave/samtools:${PATH}
          and save

          and then you can 'samtools' from wherever in terminal.

          Learning to add programs into your path (as above) is highly recommend..
          savetherhino.org

          Comment

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