Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • hmortens
    Member
    • Aug 2011
    • 12

    #1

    FASTQC setting for multiple casava fastq files?

    I am using fastqc in my workflow, but am wondering how to get the program to recognize multiple cassava fastq files, as described in the manual.
    My command for a single fastq is like:
    /fastqc –outdir=/unaligned/project_x/ --casava –noextract –quiet /unaligned/project_x/sample_13176/13176_ATCACG_L003_R1_001.fastq

    But when I use only the sample directory for the input parameter, the program does not recognize the sample directory. So, it only runs with a single fastq specified.

    Can anyone give me an example of how to run on a directory with multiple fastq files (cassava fastq)?

    I wrote to Simon Andrews but have not heard back in several days, and need to get on with it.

    Thanks.
  • mastal
    Senior Member
    • Mar 2009
    • 666

    #2
    Have you tried specifying --casava, but also listing all the files that you want analysed together, rather than just giving the name of the directory?

    Comment

    • hmortens
      Member
      • Aug 2011
      • 12

      #3
      Simon Andrews just wrote back to clarify...hoorah!
      Simple answer, but the manual is not clear on this point.
      fastqc --casava *.fastq.gz

      Running it now...

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
        by SEQadmin2




        NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
        Today, 06:46 AM
      • SEQadmin2
        Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
        by SEQadmin2



        Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

        Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
        ...
        10-14-2026, 09:55 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 10-28-2026, 09:57 AM
      0 responses
      23 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-28-2026, 09:51 AM
      0 responses
      15 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-22-2026, 11:13 AM
      0 responses
      68 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-22-2026, 10:48 AM
      0 responses
      48 views
      0 reactions
      Last Post SEQadmin2  
      Working...