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  • FASTQC setting for multiple casava fastq files?

    I am using fastqc in my workflow, but am wondering how to get the program to recognize multiple cassava fastq files, as described in the manual.
    My command for a single fastq is like:
    /fastqc –outdir=/unaligned/project_x/ --casava –noextract –quiet /unaligned/project_x/sample_13176/13176_ATCACG_L003_R1_001.fastq

    But when I use only the sample directory for the input parameter, the program does not recognize the sample directory. So, it only runs with a single fastq specified.

    Can anyone give me an example of how to run on a directory with multiple fastq files (cassava fastq)?

    I wrote to Simon Andrews but have not heard back in several days, and need to get on with it.

    Thanks.

  • #2
    Have you tried specifying --casava, but also listing all the files that you want analysed together, rather than just giving the name of the directory?

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    • #3
      Simon Andrews just wrote back to clarify...hoorah!
      Simple answer, but the manual is not clear on this point.
      fastqc --casava *.fastq.gz

      Running it now...

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