Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Starr_Hazard
    Member
    • Nov 2010
    • 19

    #1

    Chimerscan 0.4.5 runs for days and then system kills it



    I have some paired read files that range from 1.8Gb to just under 3.0Gb.
    Chimerascan works on the smallish test files distributed with the software.
    Chimerascan works on some of my files but runs for days then the system kills it

    The log files suggest that the initial runs complete through

    isize_dist.txt, breakpoint_bowtie_index.log, and tmp_singlemap_seqs.txt

    Here are the last lines of the LSF error file:

    2013-11-15 09:19:09,269 - root - INFO - Filtering encompassing chimeras with few supporting reads
    2013-11-15 09:57:34,874 - root - INFO - Extracting breakpoint sequences from chimeras
    2013-11-15 10:16:30,598 - root - INFO - Building bowtie index of breakpoint spanning sequences
    2013-11-15 10:35:32,445 - root - INFO - Extracting encompassing reads that may extend past breakpoints
    2013-11-15 10:51:26,686 - root - INFO - Separating unmapped and single-mapping reads that may span breakpoints
    2013-11-15 11:05:38,719 - root - INFO - Extracting single-mapped reads that may span breakpoints
    /home/hazards/.lsbatch/1384447987.22825.shell: line 42: 10199 Killed python /shared/app/chimerascan-0.4.5/chimerascan/chimerascan_run.py -p 8 /shared/app/BOWTIE/indexes/CHIMERASCAN_INDEXES...

    The program completes for files as large as 3.1Gb as well as smaller files near 1.8Gb but fails on many intermediate sized files.
  • luciano.cascione
    Junior Member
    • Jan 2013
    • 2

    #2
    Same problem. I split the files into 2 smaller files but the problem occurs again.

    Does someone know the reason and a possible solution?

    Bye,
    Luciano

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by SEQadmin2




      NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
      10-30-2026, 06:46 AM
    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-28-2026, 09:57 AM
    0 responses
    37 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-28-2026, 09:51 AM
    0 responses
    23 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 11:13 AM
    0 responses
    72 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 10:48 AM
    0 responses
    48 views
    0 reactions
    Last Post SEQadmin2  
    Working...