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  • Chimerscan 0.4.5 runs for days and then system kills it



    I have some paired read files that range from 1.8Gb to just under 3.0Gb.
    Chimerascan works on the smallish test files distributed with the software.
    Chimerascan works on some of my files but runs for days then the system kills it

    The log files suggest that the initial runs complete through

    isize_dist.txt, breakpoint_bowtie_index.log, and tmp_singlemap_seqs.txt

    Here are the last lines of the LSF error file:

    2013-11-15 09:19:09,269 - root - INFO - Filtering encompassing chimeras with few supporting reads
    2013-11-15 09:57:34,874 - root - INFO - Extracting breakpoint sequences from chimeras
    2013-11-15 10:16:30,598 - root - INFO - Building bowtie index of breakpoint spanning sequences
    2013-11-15 10:35:32,445 - root - INFO - Extracting encompassing reads that may extend past breakpoints
    2013-11-15 10:51:26,686 - root - INFO - Separating unmapped and single-mapping reads that may span breakpoints
    2013-11-15 11:05:38,719 - root - INFO - Extracting single-mapped reads that may span breakpoints
    /home/hazards/.lsbatch/1384447987.22825.shell: line 42: 10199 Killed python /shared/app/chimerascan-0.4.5/chimerascan/chimerascan_run.py -p 8 /shared/app/BOWTIE/indexes/CHIMERASCAN_INDEXES...

    The program completes for files as large as 3.1Gb as well as smaller files near 1.8Gb but fails on many intermediate sized files.

  • #2
    Same problem. I split the files into 2 smaller files but the problem occurs again.

    Does someone know the reason and a possible solution?

    Bye,
    Luciano

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