Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Can anyone explain....

    Hi

    Has anyone heard of a barcoding effect on the comparability of data in the bioinformatic analysis from loading a group of samples in different lanes on a HiSeq 2500? I have heard this twice now from researchers and I am not sure what they mean...nor can I find a publication discussing this...any thoughts?

    I am very new to this world (3 months into this new job) and typically at my lab we don't offer a sequencing plan or lane mapping to researchers... we load samples that have been QC multiple time thru library construction and then they go thru qPCR and get pooled then loaded for sequencing accordingly.

    I'm trying to understand why a researcher or their bioinformatician would be worried about all of their samples not being loaded into one lane or across many lanes? Or why they would be concerned and want their samples run on the sequencer in a certain way?

    Thanks in advance for your input.

  • #2
    Pooling wrong tags can potentially lead to failure of registration of clusters during the sequencing of the tag read. Illumina has specific recommendations on pooling schemes that should be considered (this example is for nextera but you will get the idea). Tech note: http://res.illumina.com/documents/pr...guidelines.pdf

    Running a large pool across several lanes/flowcells can address potential lane/run effects as opposed to running smaller pools on specific lanes.
    Last edited by GenoMax; 11-26-2013, 08:08 AM.

    Comment


    • #3
      Originally posted by GenoMax View Post
      Pooling wrong tags can potentially lead to failure of registration of clusters during the sequencing of the tag read. Illumina has specific recommendations on pooling schemes that should be considered (this example is for nextera but you will get the idea). Tech note: http://res.illumina.com/documents/pr...guidelines.pdf

      Running a large pool across several lanes/flowcells can address potential lane/run effects as opposed to running smaller pools on specific lanes.
      Thanks for your response!

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Strategies for Sequencing Challenging Samples
        by seqadmin


        Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
        03-22-2024, 06:39 AM
      • seqadmin
        Techniques and Challenges in Conservation Genomics
        by seqadmin



        The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

        Avian Conservation
        Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
        03-08-2024, 10:41 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Yesterday, 06:37 PM
      0 responses
      7 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, Yesterday, 06:07 PM
      0 responses
      7 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-22-2024, 10:03 AM
      0 responses
      49 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-21-2024, 07:32 AM
      0 responses
      66 views
      0 likes
      Last Post seqadmin  
      Working...
      X