Hi
Has anyone heard of a barcoding effect on the comparability of data in the bioinformatic analysis from loading a group of samples in different lanes on a HiSeq 2500? I have heard this twice now from researchers and I am not sure what they mean...nor can I find a publication discussing this...any thoughts?
I am very new to this world (3 months into this new job) and typically at my lab we don't offer a sequencing plan or lane mapping to researchers... we load samples that have been QC multiple time thru library construction and then they go thru qPCR and get pooled then loaded for sequencing accordingly.
I'm trying to understand why a researcher or their bioinformatician would be worried about all of their samples not being loaded into one lane or across many lanes? Or why they would be concerned and want their samples run on the sequencer in a certain way?
Thanks in advance for your input.
Has anyone heard of a barcoding effect on the comparability of data in the bioinformatic analysis from loading a group of samples in different lanes on a HiSeq 2500? I have heard this twice now from researchers and I am not sure what they mean...nor can I find a publication discussing this...any thoughts?
I am very new to this world (3 months into this new job) and typically at my lab we don't offer a sequencing plan or lane mapping to researchers... we load samples that have been QC multiple time thru library construction and then they go thru qPCR and get pooled then loaded for sequencing accordingly.
I'm trying to understand why a researcher or their bioinformatician would be worried about all of their samples not being loaded into one lane or across many lanes? Or why they would be concerned and want their samples run on the sequencer in a certain way?
Thanks in advance for your input.
Comment