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  • ampsevilla
    Junior Member
    • Mar 2023
    • 7

    #1

    training GeneMark for gene prediction

    Hi,

    I'm want to annotate a big, complex eukaryotic genome from non-model using GeneMark 4.69 gene prediction.

    I want to train GeneMark algorhytm using data from a close-related specie using its data (its genome sequence in FASTA format and its genes annotations in GFF3 and GFF format), but when I try it with using this command (or similars)
    gmes_petap.pl --ET ref/INCAR_Mch_V1.gff --sequence ref/INCAR_Mch_V1.fa > prob/predicted.gff
    I always get an error related to the format of sequence or so.

    I have got lost about how to train GeneMark from FASTA and GFF3 files. Could someone help me?

    Thank you so much.

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