Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • MissDona
    Member
    • Nov 2011
    • 17

    #1

    stange kmer content

    hi all,
    I've got this fastqc report with high ttttt all along the 1st group of paired-end reads, and high aaaaa &&. I TTTTT all along the 2nd group of paired-end reads. is it normal? thanks alot!
    (nimblegen 70M exome sequencing data, PE reads, 90bp lengh)
    Attached Files
    Last edited by MissDona; 01-04-2014, 05:55 PM.
  • MissDona
    Member
    • Nov 2011
    • 17

    #2
    other images

    as attached, thanks!
    Attached Files

    Comment

    • GenoMax
      Senior Member
      • Feb 2008
      • 7142

      #3
      There is no signal for "C". That is odd. Are all your samples showing this pattern? Were they sequenced on the same flowcell? Have you checked with your sequence provider that there was nothing technically wrong with this run?

      Comment

      • barkasn
        Junior Member
        • Mar 2012
        • 9

        #4
        This is very odd. If you can't trace the cause, consider clipping your reads.

        Comment

        Latest Articles

        Collapse

        • SEQadmin2
          Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
          by SEQadmin2



          Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

          Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
          ...
          10-14-2026, 09:55 AM
        • SEQadmin2
          New Genomics Technologies Take Aim at Long-Standing Limits
          by SEQadmin2


          Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

          We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
          ...
          09-28-2026, 10:25 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by SEQadmin2, Yesterday, 10:52 AM
        0 responses
        9 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 10-14-2026, 05:39 AM
        0 responses
        19 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 10-13-2026, 09:56 AM
        0 responses
        16 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 10-09-2026, 11:14 AM
        0 responses
        27 views
        0 reactions
        Last Post SEQadmin2  
        Working...