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  • MissDona
    Member
    • Nov 2011
    • 17

    stange kmer content

    hi all,
    I've got this fastqc report with high ttttt all along the 1st group of paired-end reads, and high aaaaa &&. I TTTTT all along the 2nd group of paired-end reads. is it normal? thanks alot!
    (nimblegen 70M exome sequencing data, PE reads, 90bp lengh)
    Attached Files
    Last edited by MissDona; 01-04-2014, 05:55 PM.
  • MissDona
    Member
    • Nov 2011
    • 17

    #2
    other images

    as attached, thanks!
    Attached Files

    Comment

    • GenoMax
      Senior Member
      • Feb 2008
      • 7142

      #3
      There is no signal for "C". That is odd. Are all your samples showing this pattern? Were they sequenced on the same flowcell? Have you checked with your sequence provider that there was nothing technically wrong with this run?

      Comment

      • barkasn
        Junior Member
        • Mar 2012
        • 9

        #4
        This is very odd. If you can't trace the cause, consider clipping your reads.

        Comment

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