pacBioToCA fails with single-end data

[Jetse]

Hello everyone,

I am trying to correct my pacbio reads with unmated solexa reads. I use the program pacbioToCA for this. After an hour I retrieve the error:

----------------------------------------START CONCURRENT Sat Jan 11 00:36:54 2014
/home/jaco001/fungiAssembly/data//tempmadurellaPacbio/runPartition.sh 1
No contig account found in bank /home/jaco001/fungiAssembly/data//tempmadurellaPacbio/asm.bnk_partition1.bnk
----------------------------------------END CONCURRENT Sat Jan 11 02:41:17 2014 (7463 seconds)
Failed to run correction job 1. Remove /home/jaco001/fungiAssembly/data//tempmadurellaPacbio/runPartition.sh to try again.

When searching on this error, I found another Thread. In this thread is mentioned there may be a problem with paired end data. I used this frg file:

{VER
ver:2
}
{LIB
act:A
acc:madurellaIllumina
ori:U
mea:0.000
std:0.000
src:
.
nft:20
fea:
forceBOGunitigger=0
isNotRandom=0
doNotTrustHomopolymerRuns=0
doTrim_initialNone=0
doTrim_initialMerBased=0
doTrim_initialFlowBased=0
doTrim_initialQualityBased=0
doRemoveDuplicateReads=0
doTrim_finalLargestCovered=0
doTrim_finalEvidenceBased=0
doTrim_finalBestEdge=0
doRemoveSpurReads=0
doRemoveChimericReads=0
doCheckForSubReads=0
doConsensusCorrection=0
forceShortReadFormat=0
constantInsertSize=0
fastqQualityValues=solexa
fastqOrientation=innie
fastqReads=/home/jaco001/fungiAssembly/data/IlluminaNoNegs.fq
.
}
{VER
ver:1
}

The orientation is unmated (ori=U), so I said to the program my input is unmated. Does someone know how I can solve this problem?

[sBeier]

Did you by any chance solve this problem? I run into the same problem while doing self-correction , so no other platform reads are involved.

[Jetse]

I did not solve this problem...