Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Jetse
    Member
    • Nov 2012
    • 38

    #1

    pacBioToCA fails with single-end data

    Hello everyone,

    I am trying to correct my pacbio reads with unmated solexa reads. I use the program pacbioToCA for this. After an hour I retrieve the error:
    ----------------------------------------START CONCURRENT Sat Jan 11 00:36:54 2014
    /home/jaco001/fungiAssembly/data//tempmadurellaPacbio/runPartition.sh 1
    No contig account found in bank /home/jaco001/fungiAssembly/data//tempmadurellaPacbio/asm.bnk_partition1.bnk
    ----------------------------------------END CONCURRENT Sat Jan 11 02:41:17 2014 (7463 seconds)
    Failed to run correction job 1. Remove /home/jaco001/fungiAssembly/data//tempmadurellaPacbio/runPartition.sh to try again.
    When searching on this error, I found another Thread. In this thread is mentioned there may be a problem with paired end data. I used this frg file:
    {VER
    ver:2
    }
    {LIB
    act:A
    acc:madurellaIllumina
    ori:U
    mea:0.000
    std:0.000
    src:
    .
    nft:20
    fea:
    forceBOGunitigger=0
    isNotRandom=0
    doNotTrustHomopolymerRuns=0
    doTrim_initialNone=0
    doTrim_initialMerBased=0
    doTrim_initialFlowBased=0
    doTrim_initialQualityBased=0
    doRemoveDuplicateReads=0
    doTrim_finalLargestCovered=0
    doTrim_finalEvidenceBased=0
    doTrim_finalBestEdge=0
    doRemoveSpurReads=0
    doRemoveChimericReads=0
    doCheckForSubReads=0
    doConsensusCorrection=0
    forceShortReadFormat=0
    constantInsertSize=0
    fastqQualityValues=solexa
    fastqOrientation=innie
    fastqReads=/home/jaco001/fungiAssembly/data/IlluminaNoNegs.fq
    .
    }
    {VER
    ver:1
    }
    The orientation is unmated (ori=U), so I said to the program my input is unmated. Does someone know how I can solve this problem?
  • sBeier
    Member
    • Jan 2013
    • 41

    #2
    Did you by any chance solve this problem? I run into the same problem while doing self-correction , so no other platform reads are involved.

    Comment

    • Jetse
      Member
      • Nov 2012
      • 38

      #3
      I did not solve this problem...

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
        by SEQadmin2



        Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

        Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
        ...
        10-14-2026, 09:55 AM
      • SEQadmin2
        New Genomics Technologies Take Aim at Long-Standing Limits
        by SEQadmin2


        Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

        We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
        ...
        09-28-2026, 10:25 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 10-22-2026, 11:13 AM
      0 responses
      28 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-22-2026, 10:48 AM
      0 responses
      20 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-15-2026, 10:52 AM
      0 responses
      38 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-14-2026, 05:39 AM
      0 responses
      44 views
      0 reactions
      Last Post SEQadmin2  
      Working...