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  • Genomics101
    Member
    • May 2012
    • 60

    #1

    Velvet 1.2.10 failing velveth (no contigs)

    Greetings!

    I have used velvet with success before, but I seem to be missing something this time.

    The data is paired-end HiSeq Illumina reads, 100bp (400bp inserts). Velvet is compiled with MAXKMERLENGTH=200, CATEGORIES=10, OPENMP=1.

    The command I am using:

    Code:
    velvet_1.2.10/velveth Assembly1 53,91,8 -shortPaired -separate -fastq HiSeq_100_R1.trimmed.fastq.pe HiSeq_100_R2.trimmed.fastq.pe
    The hash directories are formed for each kmer length, but they just come out with the following files: Log, Sequence, Roadmap -- no contigs, no stats, nothing else.

    The sequences appear to be loading, the end of the standard output is:

    Code:
    ......
    [1262.752392] Inputting sequence 84000000 / 112022250
    [1265.931838] Inputting sequence 42000000 / 112022250
    [1266.165014] Inputting sequence 28000000 / 112022250
    [1266.716441] Inputting sequence 14000000 / 112022250
    [1299.839334]  === Sequences loaded in 399.530517 s
    [1299.845389] Done inputting sequences
    [1299.845391] Destroying splay table
    [1302.434467] Splay table destroyed
    done
    Any idea what I am doing wrong?

    Thanks!
  • Genomics101
    Member
    • May 2012
    • 60

    #2
    Well, the superficial problem has been solved: I needed to run velvetg to get the rest of the information. The core issue, that I am an idiot, is harder to fix, but I appreciate everyone's patience with me. Cheers!

    Comment

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