Hello, I am new to this forum. I am writing this thread because I wanted to know what would be the best way to go about mapping RNA seq reads that I have to my reference gneome (hg 19 genome). I have 8 fastq files that I need to map. I am new to this mapping and need help.
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Basic guide: http://en.wikibooks.org/wiki/Next_Ge..._%28NGS%29/RNA
TopHat/Cufflinks: http://www.nature.com/nprot/journal/....2012.016.html
If you are a biologist wanting to learn (enough) UNIX to use some of the programs above then part I of this guide: http://korflab.ucdavis.edu/Unix_and_...rl_v3.1.1.html
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by seqadmin
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
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03-22-2024, 06:39 AM -
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by seqadmin
The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.
Avian Conservation
Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...-
Channel: Articles
03-08-2024, 10:41 AM -
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