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Hello, I am new to this forum. I am writing this thread because I wanted to know what would be the best way to go about mapping RNA seq reads that I have to my reference gneome (hg 19 genome). I have 8 fastq files that I need to map. I am new to this mapping and need help.
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Basic guide: http://en.wikibooks.org/wiki/Next_Ge..._%28NGS%29/RNA
TopHat/Cufflinks: http://www.nature.com/nprot/journal/....2012.016.html
If you are a biologist wanting to learn (enough) UNIX to use some of the programs above then part I of this guide: http://korflab.ucdavis.edu/Unix_and_...rl_v3.1.1.html -
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