Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • no qcovs output from standalone blast

    I am having trouble getting query coverage output from local/standalone blast using qcovs:

    blastn -task megablast -db nt -query seqs.fasta -max_target_seqs 5 -outfmt "7 std qcovs"

    All the other outputs seem to be fine, but I cannot get query coverage output:

    # BLASTN 2.2.26+
    # Query: comp2_c0_seq1 len=213 path=[905:0-58 1657:59-212]
    # Database: /nfs/ffeng-lab-data/blastDBs/nt
    # Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, q. start, q. end, s. start, s. end, evalue, bit score
    # 5 hits found
    comp2_c0_seq1 gi|325197263|gb|AC241982.2| 94.76 210 11 0 1210 1947 1738 2e-86 327
    comp2_c0_seq1 gi|212374812|gb|AC217674.3| 94.29 210 12 0 1210 27303 27094 8e-85 322
    comp2_c0_seq1 gi|209915647|gb|AC194652.4| 94.29 210 12 0 1210 134086 133877 8e-85 322
    comp2_c0_seq1 gi|156255299|gb|AC199643.3| 94.29 210 12 0 1210 52769 52560 8e-85 322
    comp2_c0_seq1 gi|155029613|gb|AC199644.3| 94.29 210 12 0 1210 91737 91528 8e-85 322


    Any suggesions? thanks

  • #2
    BLAST 2.2.28+ was the first release with support for custom tabular output with query coverage.

    edit. 2.2.29+ was apparently released a few days ago
    Last edited by rhinoceros; 01-20-2014, 11:49 AM.
    savetherhino.org

    Comment


    • #3
      that would make sense

      i will get the newer version

      thanks!

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM
      • seqadmin
        Strategies for Sequencing Challenging Samples
        by seqadmin


        Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
        03-22-2024, 06:39 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      31 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      33 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 09:21 AM
      0 responses
      28 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-04-2024, 09:00 AM
      0 responses
      53 views
      0 likes
      Last Post seqadmin  
      Working...
      X