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  • bio_informatics
    Senior Member
    • Nov 2013
    • 182

    #1

    help with bio perl

    Hi,
    I am handling different formats of Multiple sequence alignment files.
    I can get the format of file by

    Code:
    my $format_guess = (new Bio::Tools::GuessSeqFormat( -file => $ARGV[0]))->guess;
    but when I try to get sequence of different protein, I cannot get it.
    I have some 10 accession ids, but unable to fetch it.

    Code:
    ./perl_cript.pl file_multple_alg
    I am trying to run the script with above command in Linux, terminal
    Code:
    my $in  = Bio::AlignIO->new(-file   => $ARGV[0] ,
                                 -format => $format_guess);
    OR

    Code:
    while ( my $aln = $in->next_aln() ) {
           print $aln,"\n";
       
    }
    I get address of objects.
    I want to fetch aligned sequence of each accession/protein.

    I want to print on screen not to pass to some out file.

    Any help shall be appreciated.
    Thanks.
    Bioinformaticscally calm
  • TiborNagy
    Senior Member
    • Mar 2010
    • 329

    #2
    Try this tutorial:

    Comment

    • SES
      Senior Member
      • Mar 2010
      • 275

      #3
      The short answer is that you are printing an object, and you need to call a method on that object to get what you want. Check out the BioPerl HOWTOs for a quick overview of some of the most used parts of BioPerl.

      Comment

      • bio_informatics
        Senior Member
        • Nov 2013
        • 182

        #4
        Thank you for your help.
        Bioinformaticscally calm

        Comment

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