Transcriptomics analysis

[RickBioinf]

Hi all,

I am a newbie in the field of transcriptomics analysis and I want to get more familiar with it. I know I should first normalize (or should there also be a transformation step) the results between experiments (we're doing a comparison study) and then map the reads against the refence. What I do not understand is what kind of statistical analysis should be used and what I need to look at after mapping. I know it's good to look at the "expression values" column, but could it also be wise to sometimes look at other columns?

Any input would be helpfull.

Cheers,

RickBioinf

Ps. We have a license for the CLC Genomics Workbench.

[dnusol]

Hi RickBioinf,

I think the first thing to do could be to check on CLCbio website for publications using CLCbio

http://www.clcbio.com/support/papers/

In addition to this, I am sure CLC has some good tutorials for transcriptome analysis and their support team is willing to answer any questions.

Apart from that, a quick search on PubMed to see what others have done in similar experiments would be very useful. Just searching for 'transcriptome' would provide a good starting point.

HTH

[GenoMax]

Additionally there are four tutorials on RNA-seq analysis on this page: http://www.clcbio.com/support/tutorials/