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  • Clusters-reads correlation?

    Hi all,

    I am involved in a sequencing project using Illumina/Solexa and I'm analyzing the data. I'm a little confused because the person who did the sequencing says that they got ~16 million clusters for sequencing, when I analyze the data from the seq file I get ~35 million reads. Does this make sense or is there supposed to be a correlation between the numbers?

    Thank you!

  • #2
    Was the sequencing done paired-end? A paired-end run will produce two reads for each cluster.

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    • #3
      Yeap, I know that. The thing is that they made a mistake and they made a single-end run in a paired-end flowcell. I don't know if that is the problem...

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      • #4
        may be,I guess

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        • #5
          I would guess the first read would sequence out fine for such a case
          --
          bioinfosm

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          • #6
            Originally posted by cggj View Post
            Hi all,

            I am involved in a sequencing project using Illumina/Solexa and I'm analyzing the data. I'm a little confused because the person who did the sequencing says that they got ~16 million clusters for sequencing, when I analyze the data from the seq file I get ~35 million reads. Does this make sense or is there supposed to be a correlation between the numbers?

            Thank you!
            Can you drop here a few lines from the seq file?
            -drd

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