Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • foolishbrat
    Member
    • Nov 2008
    • 45

    Obtaining genomic features from miRNA-seq data based on UCSC hg19 alignment results

    I have miRNA-seq data (single end) which I map to the whole UCSC hg19 genome. Now given the SAM output of this mapping, what's the good pipeline to summarize that alignment so that I can get statistics of these genomic features:
    1. Unaligned
    2. Mature miRNA
    3. precursor miRNA
    4. piRNA
    5. lincRNA
    6. human Ribosomal RNA
    7. snoRNA
    8. human5S rDNA
    9. snRNA


    Namely for each of the above features how many of my reads (or percentage) are aligned?

    I know CLC-BIO or Illumina inbox software possibly already have that. But I'm looking for noncommercial and tweakable/modular way to do it.
    Last edited by foolishbrat; 01-29-2014, 05:30 AM.
  • TiborNagy
    Senior Member
    • Mar 2010
    • 329

    #2
    Try the UEA Small RNA Workbench.

    Comment

    Latest Articles

    Collapse

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 06-05-2026, 10:09 AM
    0 responses
    14 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-04-2026, 08:59 AM
    0 responses
    24 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-02-2026, 12:03 PM
    0 responses
    28 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-02-2026, 11:40 AM
    0 responses
    22 views
    0 reactions
    Last Post SEQadmin2  
    Working...