Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • bio_informatics
    Senior Member
    • Nov 2013
    • 182

    #1

    running genePRIMP

    Hi,

    I am trying to ryn geneprimp, gene prediction - validation tool. with below command line.
    I am not sure if it would run for genemark.hmm.


    ./geneprimp -if ~/Documents/file.embl.txt -it EMBL -idp 'gene ' -idlen 5 -euk N -nr ~/ -jobid NM -tool genemark.hmm -self 'neisseria meningitidis' -jobdir ~/Documents/ -blastbin ~/bin/blastOld -bl2seqbin ~/bin/blastOld

    Even I replace genemark.hmm with prodigal and the output file from tools

    I get the error:


    MSG: Can't see new qualifier in: rbs_spacer=None;score=1.11;cscore=-1.56;sscore=2.68;
    from:
    /ID=1_1;partial=10;start_type=Edge;rbs_motif=None;
    rbs_spacer=None;score=1.11;cscore=-1.56;sscore=2.68;
    rscore=0.00;uscore=3.18;tscore=0.00
    /source="Prodigal_v2.50"
    /score=1.1

    STACK: Error::throw
    STACK: Bio::Root::Root::throw Bio/Root/Root.pm:368
    STACK: Bio::SeqIO::embl::_read_FTHelper_EMBL Bio/SeqIO/embl.pm:1294
    STACK: Bio::SeqIO::embl::next_seq Bio/SeqIO/embl.pm:392
    STACK: Parser:arseGBorEMBLfile Parser.pm:263
    STACK: PAR::_run_member script/geneprimp.pl:145
    STACK: PAR::_run_member script/main.pl:26
    STACK: PAR::import /jgi/tools/lib/perl5/site_perl/5.10.1/PAR.pm:428
    STACK: __par_pl::BEGIN -e:953
    STACK: script/geneprimp.pl:0
    I have bioperl installed in my system.
    How do I resolve this error?
    Bioinformaticscally calm

Latest Articles

Collapse

  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM
  • SEQadmin2
    New Genomics Technologies Take Aim at Long-Standing Limits
    by SEQadmin2


    Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

    We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
    ...
    09-28-2026, 10:25 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 10-15-2026, 10:52 AM
0 responses
16 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-14-2026, 05:39 AM
0 responses
21 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-13-2026, 09:56 AM
0 responses
22 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-09-2026, 11:14 AM
0 responses
27 views
0 reactions
Last Post SEQadmin2  
Working...