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  • bio_informatics
    Senior Member
    • Nov 2013
    • 182

    #1

    running genePRIMP

    Hi,

    I am trying to ryn geneprimp, gene prediction - validation tool. with below command line.
    I am not sure if it would run for genemark.hmm.


    ./geneprimp -if ~/Documents/file.embl.txt -it EMBL -idp 'gene ' -idlen 5 -euk N -nr ~/ -jobid NM -tool genemark.hmm -self 'neisseria meningitidis' -jobdir ~/Documents/ -blastbin ~/bin/blastOld -bl2seqbin ~/bin/blastOld

    Even I replace genemark.hmm with prodigal and the output file from tools

    I get the error:


    MSG: Can't see new qualifier in: rbs_spacer=None;score=1.11;cscore=-1.56;sscore=2.68;
    from:
    /ID=1_1;partial=10;start_type=Edge;rbs_motif=None;
    rbs_spacer=None;score=1.11;cscore=-1.56;sscore=2.68;
    rscore=0.00;uscore=3.18;tscore=0.00
    /source="Prodigal_v2.50"
    /score=1.1

    STACK: Error::throw
    STACK: Bio::Root::Root::throw Bio/Root/Root.pm:368
    STACK: Bio::SeqIO::embl::_read_FTHelper_EMBL Bio/SeqIO/embl.pm:1294
    STACK: Bio::SeqIO::embl::next_seq Bio/SeqIO/embl.pm:392
    STACK: Parser:arseGBorEMBLfile Parser.pm:263
    STACK: PAR::_run_member script/geneprimp.pl:145
    STACK: PAR::_run_member script/main.pl:26
    STACK: PAR::import /jgi/tools/lib/perl5/site_perl/5.10.1/PAR.pm:428
    STACK: __par_pl::BEGIN -e:953
    STACK: script/geneprimp.pl:0
    I have bioperl installed in my system.
    How do I resolve this error?
    Bioinformaticscally calm

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