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  • Gangcai
    Member
    • Nov 2009
    • 30

    #1

    What does NS:i:1 means in the .sam format files?

    Hi everyone,
    I have the .sam file from tophat, and the last optional fields is NS:i:1. I have checked the sam manual, but cannot find NS Tag

    (also for XS (XS:A:-), but can be found in cufflinks manual: http://cufflinks.cbcb.umd.edu/manual.html).

    HWI-EAS244_1_1_61_293_716_0_1_6480169;1 19 chr1 1231272 255 47M76N28M = 1231188 0 ACTTCTTTTCCACGTATTTGTCCTTGATCCAGGCCTCCTTGTCCTGCCGGGAGCTGCTGGCTGTGGGTTTCCTGC P\MMY]LR\`a]a``^[FWa`]a[aa\^`a`a^\``X`X```]ZZa\V\aaaaaaa__aa_ababbabbbbaab` NM:i:1 XS:A:- NS:i:0
  • jgoecks
    Member
    • Jan 2010
    • 28

    #2
    Tophat Accepted Hits File Format Question

    Originally posted by Gangcai View Post
    Hi everyone,
    I have the .sam file from tophat, and the last optional fields is NS:i:1. I have checked the sam manual, but cannot find NS Tag
    Bump. Anyone know what this tag denotes?

    Thanks,
    J.

    Comment

    • Cole Trapnell
      Senior Member
      • Nov 2008
      • 213

      #3
      This is a non-standard SAM tag that will be deprecated in the next release of TopHat.

      Comment

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