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  • ande
    Junior Member
    • Feb 2014
    • 2

    #1

    Install Bowtie2 for mac

    I am trying to install bowtie2 using terminal. I have the unzipped folder and made it my current directory. 'make' gives this:
    make: *** No targets specified and no makefile found. Stop.

    Not sure what to do.

    thanks!
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    You do not need to "make" bowtie2 if you downloaded the pre-compiled executable file for OS X: http://sourceforge.net/projects/bowt...4.zip/download.

    You are ready to use bowtie2 right away.

    Comment

    • ande
      Junior Member
      • Feb 2014
      • 2

      #3
      so when I try the example from bowtie2:

      $BT2_HOME/bowtie2-build $BT2_HOME/example/reference/lambda_virus.fa lambda_virus

      I get an error:

      -bash: /bowtie2-build: No such file or directory

      Although bowtie2-build is in my BT2_HOME folder. Any ideas?

      Comment

      • GenoMax
        Senior Member
        • Feb 2008
        • 7142

        #4
        $BT2_HOME becomes a variable. It is not a directory name. If you actually made a folder called BT2_HOME then use the command as follows (initial $ is the system prompt (assuming you are in BT2_HOME directory):

        Code:
        $ bowtie2-build example/reference/lambda_virus.fa lambda_virus
        Can you post output of following commands from BT2_HOME directory?

        Code:
        $ pwd
        $ ls -l

        Comment

        • bio_informatics
          Senior Member
          • Nov 2013
          • 182

          #5
          bowtie2-build Genome_File outPutDirectory
          #assumed - bowtie 2 binaries are in your Path.
          Last edited by bio_informatics; 02-21-2014, 09:30 AM.
          Bioinformaticscally calm

          Comment

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