Is it possible to pipe inputs to trimmomatic. Similarly can I redirect the output to stdout ?
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In Unix-like operating systems it is possible to redirect any input or output; if the program is missing this option, simply use "/dev/stdin" and "/dev/stdout" as the filenames. The only problem is if the program needs to make multiple passes through the input or read it nonconsecutively, but I assume Trimmomatic doesn't. -
Well, the "problem", as it were, with Trimmomatic is that it wants to input 2 files (R1 pre, R2 pre) and output 4 files -- R1 post, R2 post, R1 unpaired, R2 unpaired. Given that 4 outputs are required then simple piping will not work. I have not tried it but you could probably set up FIFOs for the 4 files.Comment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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