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  • thh32
    Member
    • Feb 2014
    • 60

    #1

    Redirecting ABySS temp directory

    Basically I am filling my temp directory whilst running ABySS and due to running on a server am unable to increase the size of my temp dir but would be able to provide it with a large area if I can redirect which directory ABySS uses. I have seen online people are saying to use the -T option however this doesn't seem to exist/work in the version I am using (abyss/1.3.7). I have attempted putting the -T option at every point in the submission file but always fails. Any help anyone can provide would be greatly appreciated.

    Many thanks,
    Thomas Hitch
  • peromhc
    Senior Member
    • Sep 2009
    • 108

    #2
    in bash you can change the location of TMP like this:

    Code:
    export TMPDIR=/path/to/your/tmp
    Set TMP before you execute ABySS, and you should be OK to go.

    Comment

    • thh32
      Member
      • Feb 2014
      • 60

      #3
      @peromhc I have tried your solution but Abyss failed again and the error file stated

      Code:
      Building the suffix array...
      Building the suffix array...
      Building the suffix array...
      Building the Burrows-Wheeler transform...
      Building the character occurrence table...
      Building the Burrows-Wheeler transform...
      Building the Burrows-Wheeler transform...
      Building the character occurrence table...
      Building the character occurrence table...
      Mateless          0
      Unaligned   5004524  41.3%
      Singleton   2508903  20.7%
      FR          2159292  17.8%
      RF              307  0.00253%
      FF              887  0.00732%
      Different   2437470  20.1%
      Total      12111383
      Mateless          0
      Unaligned   4868853  25.9%
      Singleton   4053627  21.5%
      FR          3480448  18.5%
      RF              321  0.00171%
      FF              708  0.00376%
      Different   6418481  34.1%
      Total      18822438
      sort: write failed: /tmp/725829.1.large.q/sortQGku1s: No space left on device
      error: no @SQ records in the SAM header
      make: *** [L002-3.dist] Error 1
      make: *** Deleting file `L002-3.dist'
      make: *** Waiting for unfinished jobs....
      Mateless          0
      Unaligned   4817815  25.8%
      Singleton   4157786  22.3%
      FR          3414023  18.3%
      RF              275  0.00147%
      FF              650  0.00348%
      Different   6260889  33.6%
      Total      18651438

      Comment

      • westerman
        Rick Westerman
        • Jun 2008
        • 1104

        #4
        Could you show us your EXPORT command followed by your ABySS command? If done properly then the program 'sort' (which ABySS is using) should not point to '/tmp'.

        Comment

        • thh32
          Member
          • Feb 2014
          • 60

          #5
          I used the command in the wrong place, now it works. I had put the export command separate to my submission script, when export command was put into the submission file it redirected the tmpdir and works fine.

          Thanks alot,

          Tom

          Comment

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