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  • seq-seeker
    Junior Member
    • Apr 2014
    • 2

    freebayes

    Hi,
    I am using FreeBayes for SNP calling. When I input my sequences Freebayes runs fine until it hits a part of the sequence with a lot of NNNNs. I get the following error (I edited out a few Ns for this post):

    Exception: Cannot read past the end of the alignment's sequence.
    CTGGCAGCCTCTGGTTATCTTTAGTTTCTTCACTCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN\NNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTGGATCGAAACGAAACAGCCGATTGTTGTTTTCTTTATCGCAAGGATGATGAAGAAACTTTGGGAGAGAAACAAGTGAAGCCCGTTGGTCTAGCAAGTGATTGTAAAATG
    ATCAAGACCCCACAAGGGAAAACAATCCTTAAAGCAGACTTGAGATCGATCATACC\GCAAGTGATTGTAAAATGT
    Chr1:32635
    Aborted

    Here is the input I used:

    freebayes -f reference.fa sorted.bam > sorted.vcf

    I ran a trial with a truncated data set of just the first 100 lines of input, which ran fine and produced the expected out file in vcf format.

    Does anyone have an idea how to handle this?
    Thanks.
  • AdrianP
    Senior Member
    • Apr 2011
    • 130

    #2
    I have NNNN in my sequences and it runs just fine.

    Is that a "\" i see in your sequence?

    Comment

    • seq-seeker
      Junior Member
      • Apr 2014
      • 2

      #3
      Adrian,
      I checked the \. They are not in the sequences and must have been introduced when I shortened the sequence while copying the error message into the window.

      Comment

      • AdrianP
        Senior Member
        • Apr 2011
        • 130

        #4
        and it is talking about alignment, so I believe the error relates to the .bam file?

        Comment

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