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  • Jocelyn SP
    Junior Member
    • May 2014
    • 1

    finding SNPs on PlasmoDB

    I have a set of aligned sequences for Plasmodium falciparum, and I want to confirm what strain it is. I have used SNVerIndividual to get a list of SNPs. Now I would like to compare them with known SNPs in the MSP1 region. I can do it on PlasmoDB in a clumsy, visual way, by opening the genome browser for part of the region, having a SNP track, hovering on a SNP diamond and clicking through to the SNP entry to find the distribution of alleles, but obviously I would rather not!
    PlasmoDB has a few searches that look right, e.g. 'Identify SNPs based on Genomic Location (Sanger Sequencing)' but I can't get them to work. I either get all the SNPs in the chromosome, or none.
    Any advice?

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
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