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  • ilovekk1989
    Junior Member
    • Feb 2014
    • 1

    #1

    using Breakdancer for mate pair

    Dear all ,

    I have a little problem about BreakDancer.

    I have a mate-pair data from Illumina , and I use some tools to reverse and trim each read.

    now I want to use Breakdancer to find discordant reads ,

    and I use this command :

    perl BreakDancerMax.pl -l -f -o Chr6 5k.sorted.config > 5k_sorted.l.chr6.max

    I want to know should I use option "-l" .

    Because if I use it , breakdancer will find so many insertions and their size are about 3500.

    If I wont use -l , breakdancer still find structural variations but total is lower than I use -l .

    thank you guys.

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