Dear all ,
I have a little problem about BreakDancer.
I have a mate-pair data from Illumina , and I use some tools to reverse and trim each read.
now I want to use Breakdancer to find discordant reads ,
and I use this command :
perl BreakDancerMax.pl -l -f -o Chr6 5k.sorted.config > 5k_sorted.l.chr6.max
I want to know should I use option "-l" .
Because if I use it , breakdancer will find so many insertions and their size are about 3500.
If I wont use -l , breakdancer still find structural variations but total is lower than I use -l .
thank you guys.
I have a little problem about BreakDancer.
I have a mate-pair data from Illumina , and I use some tools to reverse and trim each read.
now I want to use Breakdancer to find discordant reads ,
and I use this command :
perl BreakDancerMax.pl -l -f -o Chr6 5k.sorted.config > 5k_sorted.l.chr6.max
I want to know should I use option "-l" .
Because if I use it , breakdancer will find so many insertions and their size are about 3500.
If I wont use -l , breakdancer still find structural variations but total is lower than I use -l .
thank you guys.