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Best Aligner for Duplicated Sequences
I'm looking around for some input from fellow SEQers. I'm experimenting with different aligners. I'm looking for an aligner that does a good job of correctly mapping reads from duplicated sequences, while at the same time is used fairly commonly. I am already looking into bwa, bowtie, and snap, but I would like to add one more to the list. Any suggestions? Would novoalign or stampy be good choices?
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i think you should change the query to aligner for multiply mapped reads!
most aligners you mention have different options to select multiply mapped reads, so it might end up being multiple options from the same tool as well...--
bioinfosm -
Thanks bioinfosm! I'll look into that. Do you have any experience with stampy or novoalign?Comment
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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