addSolexaReads.perl error

[wisojo]

Hey guys,

I'm trying to use the command addSolexaReads.perl to add the rest of my fastq reads to an assembly made with Newbler, but I keep getting the error "-ace must be specified." I think I'm including all of the correct files for the addSolexaReads.perl command but still keep getting that error.

Has anyone come across this before or might know where the problem is coming from?

Thanks!

[Danos2000]

Syntax has changed

The addSolexaReads.perl syntax changed around consed 23.

Old syntax:

addSolexaReads.perl your_ace_file.ace your_fileoffilenames.fof your_fasta.fasta

New syntax, where you explicitly specify the options:

addSolexaReads.perl -ace your_ace_file.ace -fastqfof your_fileoffilenames.fof -fasta your_fasta.fasta

More:

https://vimeo.com/70137766

--Dan