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  • dpryan
    Devon Ryan
    • Jul 2011
    • 3478

    #31
    Yeah, they'll take a while to get generated.

    Comment

    • simobioinfo
      Member
      • Aug 2014
      • 40

      #32
      Hi... now 24 hours are passed and indexing is still running on my MAc (see previous post of this thread). What can I do now? My processor is dual core... I have to insert an option into the command line to take into account that processor has 2 core?

      Comment

      • dpryan
        Devon Ryan
        • Jul 2011
        • 3478

        #33
        24 hours seems excessive. Can you see it still using one of your cores? I don't think the indexing can be run multi-core. What species are you trying to index? Perhaps one of us can just create and post the indices so you can download them.

        Comment

        • GenoMax
          Senior Member
          • Feb 2008
          • 7142

          #34
          simobioinfo is indexing GRCh37/human. If bandwidth is not a problem then you can download the preformatted bundle (sequence, indexes and annotations) from this link: http://support.illumina.com/sequenci...e/igenome.html

          @simobioinfo: This is the sort of thing I was trying to warn you about in terms of user experience.

          @Devon: If you have indexes for that known to work on a Mac you could post them for simobioinfo.

          Comment

          • dpryan
            Devon Ryan
            • Jul 2011
            • 3478

            #35
            I'll give it a whirl on my macbook pro, but it's rather old and low on RAM. Assuming that doesn't work, on Tuesday I'll be back in the office and just use one of our beefier Macs if this isn't resolved by then.

            Comment

            • biocomputer
              Member
              • Dec 2013
              • 62

              #36
              Originally posted by simobioinfo View Post
              Hi... now 24 hours are passed and indexing is still running on my MAc (see previous post of this thread). What can I do now? My processor is dual core... I have to insert an option into the command line to take into account that processor has 2 core?
              How much RAM does this Mac have? If you look at my previous post you'll see where I said that if you don't have enough RAM it will take very, very long to build in the index. To build the human genome you probably need 5-6GB but you will have to monitor RAM usage as the index is building to know for sure. And it doesn't matter how many cores you have Bowtie2-build is a single process.

              Comment

              • simobioinfo
                Member
                • Aug 2014
                • 40

                #37
                Originally posted by biocomputer View Post
                How much RAM does this Mac have? If you look at my previous post you'll see where I said that if you don't have enough RAM it will take very, very long to build in the index. To build the human genome you probably need 5-6GB but you will have to monitor RAM usage as the index is building to know for sure. And it doesn't matter how many cores you have Bowtie2-build is a single process.
                My mac has 4Gb of RAM. I suppose that it could be enough to build index. I don't know how to check ram usage.

                Comment

                • dpryan
                  Devon Ryan
                  • Jul 2011
                  • 3478

                  #38
                  I was able to build the index on another machine and use it on my macbook pro with 3 gigs of RAM. I really wouldn't recommend doing this on a machine with that little RAM as you end up swapping all the time. Since you have more RAM than my old laptop, hopefully you'll get better performance. I'll post the indices later today and send you a link.

                  Comment

                  • dpryan
                    Devon Ryan
                    • Jul 2011
                    • 3478

                    #39
                    @simbioinfo: I just sent you a PM with some links to the bowtie2 indices that worked on my Mac. Let me know if they work for you.

                    Comment

                    • simobioinfo
                      Member
                      • Aug 2014
                      • 40

                      #40
                      Thank you everybody. I was on vacation. I will try soon indexes you sent me

                      Comment

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