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  • dpryan
    replied
    This was also cross-posted on Biostars, where Jeremy Leipzig and I both already replied. Please don't cross-post, it creates double work.

    Leave a comment:


  • GenoMax
    replied
    You are working with a very uncommon genome so this is not going to be a trivial task. You are going to have to start blasting the sequences and doing some legwork to assign a putative function to the proteins you have identified as DE.

    You can find the protein sequence for the gene models at the link Devon had posted yesterday: http://marinegenomics.oist.jp/genome...s?project_id=3

    Only 13 proteins from Acropora appear to be in RefSeq: http://www.ncbi.nlm.nih.gov/protein?...from_uid=10529

    Leave a comment:


  • Determining the biological process or function of genes

    I have a list of DEGs from DESeq from A.digitifera coral. I put them into DAVID software as a list, selected Identifier as "OFFICIAL_GENE_SYMBOL", chose Gene List, and then submitted the list. Then, I chose "Option 1: Convert the gene list to DAVID". None of the genes were in the DAVID database, was what was indicated as the result.

    Is there any software I could use that might be better for my species? I just need to do this (very) quick and dirty (for now). I am interested to determine biological processes or functions of these DEGs I have. Here is a sublist of the DEGs, in case:

    (I appreciate any help!!!!)

    aug_v2a.00154.t1
    aug_v2a.00307.t1
    aug_v2a.00601.t1
    aug_v2a.00603.t1
    aug_v2a.00823.t1
    aug_v2a.00990.t1
    aug_v2a.01279.t1
    aug_v2a.01408.t1
    aug_v2a.01492.t1
    aug_v2a.01496.t1
    aug_v2a.01618.t1
    aug_v2a.02238.t1
    aug_v2a.02239.t1
    aug_v2a.02459.t1
    aug_v2a.03293.t1
    aug_v2a.03318.t1
    aug_v2a.03417.t1
    aug_v2a.03732.t1
    aug_v2a.03955.t1
    aug_v2a.04041.t1
    aug_v2a.04353.t1
    aug_v2a.04482.t1
    aug_v2a.04679.t1
    aug_v2a.04822.t1
    aug_v2a.05042.t1
    aug_v2a.05093.t1
    aug_v2a.05179.t1
    aug_v2a.05185.t1
    aug_v2a.05514.t1
    aug_v2a.05561.t1
    aug_v2a.05667.t1
    aug_v2a.05723.t1
    aug_v2a.05776.t1
    aug_v2a.05823.t1
    aug_v2a.05859.t1
    aug_v2a.05896.t1
    aug_v2a.05945.t1
    aug_v2a.06125.t1
    aug_v2a.06180.t1
    aug_v2a.06372.t1
    aug_v2a.06390.t1
    aug_v2a.06468.t1
    aug_v2a.06469.t1
    aug_v2a.06517.t1
    aug_v2a.06763.t1
    aug_v2a.06991.t1
    aug_v2a.07140.t1
    aug_v2a.07189.t1
    aug_v2a.07227.t1
    aug_v2a.07393.t1
    aug_v2a.07523.t1
    aug_v2a.07683.t1
    aug_v2a.07824.t1
    aug_v2a.08028.t1
    aug_v2a.08251.t1
    aug_v2a.08395.t1
    aug_v2a.08398.t1
    aug_v2a.08439.t1
    aug_v2a.08791.t1
    aug_v2a.08951.t1
    aug_v2a.09028.t1
    aug_v2a.09216.t1
    aug_v2a.09226.t1
    aug_v2a.09253.t1
    aug_v2a.09307.t1
    aug_v2a.09322.t1
    aug_v2a.09376.t1

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  • seqadmin
    Exploring the Dynamics of the Tumor Microenvironment
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    The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...
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